Pharma Column Selection Matrix
Expert Decision Support System & Method Suitability Guardrails
USP <1224> Compliant
🧬 Analyte Characterization
Target Molecule Mass (Size)
Small Molecule (< 1,500 Da)
Biologics / Large Macromolecules (> 1,500 Da)
Calculated Partition Value (log P)
2.0
Charge State / Ionization Mode
Neutral Compounds (No Ionization)
Weak Base (e.g., Alkylamines, Pyridines)
Weak Acid (e.g., Carboxylic Acids, Phenols)
Strong Ionics / Quaternary Ammonium
Pi-Electron Cloud Geometry
Aliphatic / Low-Aromatic Footprint
Highly Aromatic / Halogenated Regioisomers
Requires Chiral Separation (Enantiomers)
🧪 Target Mobile Phase
Planned Mobile Phase pH
4.5
Starting Aq Buffer Fraction
50%
Mechanistic Separation Space Map
HILIC / ION-EXCHANGE ZONE
REVERSED PHASE (C18 / C8)
POLAR EMBEDDED / AQ PHASE
AROMATIC Pi-Pi (PFP / PHENYL)
Polar (-3.0)
Log P = 1.5
Non-Polar (+6.0)
Recommended Stationary Chemistry
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Regulatory
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Base Particle Substrate Support
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Primary Mechanistic Separation Mode
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Method Development Rationale
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